Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERISWNQYFMAQSHLLALRSTCPRLSVGATIV-RDKRMIAGGYNGSIAGGVHCADEGCLMI----DDHCARTIHAEMNAILQCSKFGVPTDGAEIYVTHYPCIQCCKSIIQAGIKTVYYAEDYKTHP----YAQELFEQAGVTVEQVELDEMIVDLKNREKLSFVAGLIGKLADAGLAEEELKKIHEQANTLFTSYV 2W4L Chain:A ((8-166)) -DYLEWPEYFMAVAFLSAQRSKDPNSQVGACIVNSENKIVGIGYNGMPNGCSDDVLPWRRTAENKLDTKYPYVCHAELNAIMN------DVKGCSMYVALFPCNECAKLIIQAGIKEVIFMSDKYHDSDEATAARLLFNMAGVTFRKFIP----------KCSKIVIDFDSI--------------------------

General information: TITO was launched using: RESULT: Template: 2W4L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 696 -56849 -81.68 -389.38 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -81.68 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_2W4L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W4L-query.scw PDB file : Tito_Scwrl_2W4L.pdb: