Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDSNLTNPIKAFFHDEFPEQYQEPPGLQKNMKPVPDCGEKSYKGSGKLTGRKALVTGGDSGIGRAAAIAYAREGADVAINYLPEEQPDAEEVKELIEAEGRKAVLIPGDLSDESFCQDLVKQSHHELGGLDVLALVAGKQQAVENIEDLPTEQIYKTFEVNVFSLYWVVKAALPYLPEGAS--IITTTSVEG-YNPSPMLLDYAATKNAIIGFTVGLGKQLASKGIRVNSVAPGPIWTPLQISGGQPTENIPKFGQGTPPAPLNRAGQPVELADVYVFLASENSSYVTSQVYGITGGIPTA 3PK0 Chain:C ((8-250)) ------------------------------------------------LQGRSVVVTGGTKGIGRGIATVFARAGANVAVAGRSTADIDA-CVADLDQLGSGKVIGVQTDVSDRAQCDALAGRAVEEFGGIDVVCANAGVFPDAP-LATMTPEQLNGIFAVNVNGTFYAVQACLDALIASGSGRVVLTSSITGPITGYPGWSHYGATKAAQLGFMRTAAIELAPHKITVNAIMPGNIMTEGLLENGE--EYIASMARSIPAGAL---GTPEDIGHLAAFLATKEAGYITGQAIAVDGG----

General information: TITO was launched using: RESULT: Template: 3PK0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1359 -147836 -108.78 -615.98 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -108.78 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_3PK0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PK0-query.scw PDB file : Tito_Scwrl_3PK0.pdb: