Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNVLQTTNLSKTYYSNKGTISYQALSAFDLSVSKGEFVGIMGPSGSGKTTLLNLLATIDKPTQGEMMINGIQPKTLKDQELAL----FRRRELGFVFQDFNLLDTLTIRENILLP---LALDKV----KLREMEARLDELADTLQIKHILDHRTYEVSGGQQQRAACARAIIHNPALILADEPTGNLDSKSAKQVMNTLAQLNEEKEATILLVTHDATAASFCKRIVFIKDGRFFSEIHRGTNRQVFYQSILDTLSVLGGDFHEFENYRP 2PMK Chain:A ((21-220)) ------------------------LDNINLSIKQGEVIGIVGRSGSGKSTLTKLIQRFYIPENGQVLIDG--------HDLALADPNWLRRQVGVVLQD-NVLLNRSIIDNISLANPGMSVEKVIYAAKLAGAHDFISELREGYNT--IVGEQGAGLSGGQRQRIAIARALVNNPKILIFDEATSALDYESEHVIMRNMHKIC--KGRTVIIIAHRLSTVKNADRIIVMEKGKIVEQ---------------------------------

General information: TITO was launched using: RESULT: Template: 2PMK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 850 -19926 -23.44 -105.43 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -23.44 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_2PMK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PMK-query.scw PDB file : Tito_Scwrl_2PMK.pdb: