Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFGNVSTAMITPFDNKGNVDFQKLSTLIDYLLKNGTDSLVVAGTTGESPTLSTEEKIALFEYTVKEVNG-RVPVIAGTGSNNTKDSIKLTKKAEEAGVDAVMLVTPYYNKPSQEGMYQHFKAIAAETSLPVMLYNVPGRTVASLAPETTIRLAADIPNVVAIKEASGDLEAITKIIAETPEDFYVYSGDDALTLPILSVGGRGVVSVASHIAGTDMQQMIKNYT-NGQTANAALIHQKLLPIMKELFKAPNPAPVKTALQLRGL-DVGSVRLPLVPLTEDERLSLSSTISEL 5F1U Chain:C ((10-275)) -------TALITPFKN-GKVDEQSYARLIKRQIENGIDAVVPVGTTGESATLTHEEHRTCIEIAVETCKGTKVKVLAGAGSNATHEAVGLAKFAKEHGADGILSVAPFYNKPTQQGLYEHYKAIAQSVDIPVLLYNVPGRTGCEISTDTIIKLFRDCENIYGVKEASGNIDKCVDLLAHEPRMMLI-SGEDAINYPILSNGGKGVISVTSNLL-PDMISALTHFALDENYKEAKKINDELYNINKILFCESNPIPIKTAMYLAGLIESLEFRLPLC-----------------

General information: TITO was launched using: RESULT: Template: 5F1U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1499 -131673 -87.84 -500.66 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : -87.84 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_5F1U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F1U-query.scw PDB file : Tito_Scwrl_5F1U.pdb: