Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENVFDYEDIQLIPAKCIVNSRSECDTSVRLGGH-TFKLPVVPANMQTIIDEKLAISLAENGYFYVMHR-FEPETRID-FIKDMNARGLFSSISVGVKDEEYEFVRQLAEENLTPEYVTIDIAHGHSNAVIEMIQHLKKHLPDSFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASK---PIIADGGIRTHGDIAKSIRFGATMVMIGSLFAGHEESPGQTIEKDGKLYKEYFGSAS-------EFQKGEKKN--VEGKKMHVAHKGSIKDTLIEMEQDLQSSISYAGGTKLNAI-RNVDYVIVKNSIFNGDKY 4IXH Chain:C ((13-340)) ----LTFEDILLVPNYSEVLPR-EVSLETKLTKNVSLKIPLISSAMDTVTEHLMAVGMARLGGIGIIHKNMDMESQVNEVLKVKNSGGLRVGAAIGVN--EIERAKLLVEAGV--DVIVLDSAHGHSLNIIRTLKEIKSKM-NIDVIVGNVVTEEATKELIENGADGIKVGIGPGSICTTRIVAGVGV--PQITAIEKCSSVASKFGIPIIADGGIRYSGDIGKALAVGASSVMIGSILAGTEESPGEKELIGDTVYKYYRGMGSVGAMKSG---------MVPEGIEGRVKYKGEMEGVVYQLVGGLRSCMGYLGSASIEELWKKSSYVEITTS-------

General information: TITO was launched using: RESULT: Template: 4IXH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1827 -18225 -9.98 -61.16 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -9.98 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_4IXH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IXH-query.scw PDB file : Tito_Scwrl_4IXH.pdb: