Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEMMGMENIQQNQGLKQKDEQFVWHAMKGAHQADSLIAQKAEGAWVTDTDGRRYLDAMSGLW-CVNIGYGRKELAEAAYEQLKELPYYP-LTQSHAPAIQLAEKLNEWLGGD--YVIFFSNSGSEANETAFKIARQYHLQNGDHSRYKFISRYRAYHGNTLGALSATGQAQRKYKYEPLSQGFLHAAPPDIYRNPDDADTLESANEIDRIMTWELSETIAGVI----MEPIITGGGILMPPDGYMKKVEDICRRHGALLICDEVICGFGRTGEPFGFMHYGVKPDIITMAKGITSAYLPLSATAVKRDIFEAYQGEAPYDRFRHVNTFGGSPAACALALKNLQIMEDEQLIQRSRDLGAKLLGELQAL-REHPAVGDVRGKGLLIGIELVKDKLTKEPADAAKVNQVVAACKEKGLI---IGKNGDTVAGYNNVIQLAPPFCLTEEDLSFIVKTVKESFQTI 2ZUK Chain:B ((46-438)) -----------------------------------------RGARLIEENGRELID-LSGAWGAASLGYGHPAIVAAVSAAAANPAGATILSASNAPAVTLAERLLASFPGEGTHKIWFGHSGSDANEAAYRAIVKATGRSG------VIAFAGAYHGCTVGSMAFSGH----------ADGLILLPYPDPYR-PYRND--PTGDAILTLLTEKLAAVPAGSIGAAFIEPIQSDGGLIVPPDGFLRKFADICRAHGILVVCDEVKVGLARSGRLHCFEHEGFVPDILVLGKGLGGGLP-LSAVIAPAEILDCASAFA-------MQTLHGNPISAAAGLAVLETIDRDDLPAMAERKGRLLRDGLSELAKRHPLIGDIRGRGLACGMELVCDRQSREPAR-AETAKLIYRAYQLGLVVYYVGMNG-------NVLEFTPPLTITETDIHKALDLLDRAFSEL

General information: TITO was launched using: RESULT: Template: 2ZUK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2167 -218495 -100.83 -585.78 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -100.83 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_2ZUK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZUK-query.scw PDB file : Tito_Scwrl_2ZUK.pdb: