Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAGGIWTEDASRMEAM-SLDYFLSYPRLFWPKFKELFQMKMSGSFEPNEGHLLLAELEKQGKQVDIFTQNIDGLHKKAG--SRHVYELHGSIQTAACPACGARYD----LPHLLEREVPECTAAGNNGDICGTVLKTDVVLFGDAV-MHFDTLYEKLDQADLLLVIGTSLEVAPARFVPEDASLIPGMKKVIINLEPTYCDSLFDMVIHQKIGEFARSLGMKK 4C78 Chain:A ((23-211)) -----------QRVVVMVGAGISTPSGIPDFRS-------DLPYPEAIFELPFFFHNPKPFFTLAKELY----PGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCP-------VCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEP-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4C78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 753 -73002 -96.95 -431.96 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -96.95 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_4C78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C78-query.scw PDB file : Tito_Scwrl_4C78.pdb: