Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNKIYDWVDERLDITPMWRDIADHEVPEHVNPAHHFSAFVYCFGGLTFFVTVIQVLSGMFLTMYYVPDIKNAWESVYYLQNEVAFGQIVRGMHHWGASLVIVMMFLHTLRVFFQGAYKKPRELNWIVGVLIFFVMLGLGFTGYLLPWDMKALFATKVGLQIAEATPLIGTQVKTLLAGHPDIVGAQTLTRFFAIHVFFLPAALFGLMAAHFIMIRKQGISGPL 4OGQ Chain:A ((2-215)) --ANVYDWFEERLEIQAIAEDVTSKYVPP-----HV--NIFYCLGGITLVCFLIQFATGFAMTFYYKPTVAEAYSSVQYIMNEVNFGWLIRSIHRWSASMMVLMMILHVFRVYLTGGFKKPRELTWVSGVILAVITVSFGVTGYSLPWDQVGYWAVKIVSGVPEAIPVVGVLISDLLRGG-SSVGQATLTRYYSAHTFVLPWLIAVFMLFHFLMIRKQGISGPL

General information: TITO was launched using: RESULT: Template: 4OGQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 677 -114443 -169.04 -534.78 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -169.04 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.295

(partial model without unconserved sides chains): PDB file : Tito_4OGQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OGQ-query.scw PDB file : Tito_Scwrl_4OGQ.pdb: