Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGGMALTGNKAVVYKGKG-TVAVEDIGYPELILRDGPGVPKANVNRKCEHGVILKVITTNICGSDQHMVRGRTT-APEGLVLGHEITGEVIETGRDVEFIKKGDIVSVPFNIACGRCVMCKTQKTHVCLNVNPDRPGS---------------AYGYVDMGGWVGGQSEYVMVPYADFQLLVFPDKEQALEKILDLTMLSDIFPTGFHGAYT-AGVQTGSTVYIAGAGPVGLAAAHSAQLLGASTVIVGDLNEDRLAQARSFGC-ETVNVQKHD-RLGEQIEQILGEPTVDAAVDCVGFEASGHGNQGEAPAAVLNSIMDVTQV-GGSLGIPGLYVTEDPGAKDADAKTGSLKIRFGLGWAKAHTFVTG-QTP--AMTYNRNLMKAILSGRAQIAKAVNATVISLDDAPKGYSDFDKGAAKKFVIDPHGTLK 2FZW Chain:A ((3-364)) ----EVIKCKAAVAWEAGKPLSIEEIEVAPPK----------------AHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHLGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHY----MGTSTFSEYTVVADIS--VAKIDPL----APLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMT-DGGVDYSFECIGNV------------KVMRAALEACHKGWGVSVVVGVAASGE-----------EIATRPFQ-LVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLS-FDEINKAFELMHSG--------------

General information: TITO was launched using: RESULT: Template: 2FZW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2100 -40960 -19.50 -121.18 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -19.50 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_2FZW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FZW-query.scw PDB file : Tito_Scwrl_2FZW.pdb: