Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPEMEAIGLKDVHYLPSNGYLVGTLPATSDRLCHKIGFISHMDTA-DFNAENITPQI-VDYKGGDIELGDS-GYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASHPEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLELTFEGRNVHPGTAKNQMINALQLAMDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQAKSSYIIRDFEEVNFLKRKHLAQDIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTPINIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTMEKAVDVILGIVAKD 3IFE Chain:A ((27-429)) ---EELIERFTRYVKIDTQSNEDSHTVPTTPGQIEFG-KLLVEELKEVGLTEV-TMDDNGYVMATLPANTDKDVPVIGFLAHLDTATDFTGKNVKPQIHENFDGNAITLNEELNIVLTPEQFPELPSYKGHTIITTDGTTLLGADDKAGLTEIMVAMNYLIHNPQIKHGKIRVAFTPDEEIGRGPAHFDVEAFGASFAYMMDGGPLGGLEYESFNAAGAKLTFNGTNTHPGTAKNKMRNATKLAMEFNGHLPVEEAPEYTEGYEGFYHLLSLNGDVEQSKAYYIIRDFDRKNFEARKNTIENIVKQMQEKYGQDAVVLEMNDQYYNMLEKIEPVREIVDIAYEAMKSLNIEPNIHPIRGGTDGSQLSYMGLPTPNIFTGGENYHGKFEYVSVDVMEKAVQVIIEIARRF

General information: TITO was launched using: RESULT: Template: 3IFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -144401 for 3434 contacts (-42.1/contact) + 2D Compatibility (PS) -43879 + (NN) -29120 + (LL) 492 1D Compatibility (HY) -37600 + (ID) 10600 Total energy: -265108.0 ( -77.20 by residue) QMean score : 0.604

(partial model without unconserved sides chains): PDB file : Tito_3IFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IFE-query.scw PDB file : Tito_Scwrl_3IFE.pdb: