Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLKSKLFVICLAAAAIFTAAGVSANAEALDFHVTERGMT 2PQ4 Chain:B ((1-35)) MKLSRRSFMKANAVAAAAAAAGLSVPGVARA--VVGQ---

General information: TITO was launched using: RESULT: Template: 2PQ4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 45 -4628 -102.84 -132.23 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -102.84 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.183

(partial model without unconserved sides chains): PDB file : Tito_2PQ4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PQ4-query.scw PDB file : Tito_Scwrl_2PQ4.pdb: