Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAPKDVRALIREGKINGPTAGMSGGYAQANLVVLKKDLAFDFLLFCQRNQKPCPVLDVTEAGSPVPSLAAPDADIRTDFPKYRIYRHGILTEEVSDITPYWED--DFVGFLIGCSFSFEQALINNGIAVRHIDEGTNVSMYKTNIDCVPAGAFHGQMVVSMRPVPERLAVRAAQVTSRFPAVHGGPIHIGNPGAIGIRDLGKPDFGDAVSIRDGEVPVFWACGVTPQAVAMNVKPEMVITHAPGHMLITDIRDESLAVL 2PIF Chain:A ((14-262)) -AAREARGTYRNGL-VTPTAGVAPGMTQANLIALPRDWAYDFLLYAQRNPKACPILDVSDAGSP-TTLLAEGSDLRTDIPMYRIWRDGKLAEEVSDATQAWAEHDDMVAFLIGCSFTFETPLQEAGIEVRHITDGCNVPMYRTNRACRPAGRLHGEMVVSMRPIPADRVAEASAISGR----HGAPVHIGEPGRLGINDLSRPDFGDAVSIKPGEVPVFWACGVTPQAAVMASGVPFAITHSPGYMFITDVP-------

General information: TITO was launched using: RESULT: Template: 2PIF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1412 -153235 -108.52 -630.59 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -108.52 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_2PIF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PIF-query.scw PDB file : Tito_Scwrl_2PIF.pdb: