Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDITFNTGLMGSGKSKKLID-DYLIDPK-EKVALSVSLTEDTFSRG--KIESRDGRSLRSINLNRDQFKQNISLLEII---IFMTNTQTIYIDESQFLPKETVEKFVSLSESYHVPIHFYGLDLTFTGELFDSSSHLLTILPSENINRISRGCEASKCSKIAQYNARIVDGKVSR-SGETFVEEKSY-YLALCSDHYYNDEKII 2B8T Chain:A ((12-199)) GWIEFITGPMFAGKTAELIRRLHRLEYADVKYLVFK---PKIDTRSIRNIQSRTGTSLPSVEVES-AP----EILNYIMSNSFNDETKVIGIDEVQFFDDR-ICEVANILAENGFVVIISGLDKNFKGEPFGPIAKLFT--YADKITKLTAICNE--CGAEATHSLRKIDGKHADYNDDIVKIGCQEFYSAVCRHHHKVPN---

General information: TITO was launched using: RESULT: Template: 2B8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 867 -67478 -77.83 -376.97 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -77.83 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.378

(partial model without unconserved sides chains): PDB file : Tito_2B8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B8T-query.scw PDB file : Tito_Scwrl_2B8T.pdb: