Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MPWSMKDYPASLKNLEKPVRKKAIDIANAMIDEG--YEEGRAIPIATSKAKEWAENASTDEIDDFLTHDDETERDADPSSGSGPELMNKAEHVIKH--KKGWAVKAEGAKRVSEIKDTKKEAIERAKEIAAHKGTEVIVHLADGSVQRKIKTGS 4J1V Chain:A ((9-180)) LRQAVMLPEGEDLNEWIAVNTVDFFNQINMLYGTITEFCTEASCPVMSAGPRYEYHWADPIKCS-APKYIDYLMTWVQDQL---DDETLFPSKIGVPFPKNFMSVAKTILKRLFRVYAHIYHQHFDSVMQLQEEAHLNTSFKHFIFFVQEFNLIDRRELAPLQELIEKLG

General information: TITO was launched using: RESULT: Template: 4J1V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 521 -34746 -66.69 -237.98 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -66.69 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_4J1V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J1V-query.scw PDB file : Tito_Scwrl_4J1V.pdb: