Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MD------NKELQKLTEDISETYFKKPFR-----HQALFNDRLKTTGGRYLLTSHNIELNRKYLIEHGREELIGII-KHELCHYHLHLEGKGYKHRDRDFRMLLQQVNAPRFCTPLKKKAENKKTYMYICTTCGQQYIKKRAMNPDRYR--CGKCRGKIKRIFS-- 1ZWT Chain:A ((1-158)) MEQSASDSNKSQNAISEVMSATSAINGLYIGQTSYSGLDSTILLNTSA--IPDNYKDTTNKKITNPFGGELNVGPANNNTAFGYYLTLTRLD-KAACVSLATLNLGTSAKGYGVNISGENNITS-----FGNSADQAAKSTAITPAEAATACKNTDSTNKVTYFMK

General information: TITO was launched using: RESULT: Template: 1ZWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 651 -1449 -2.23 -10.20 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -2.23 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.113

(partial model without unconserved sides chains): PDB file : Tito_1ZWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZWT-query.scw PDB file : Tito_Scwrl_1ZWT.pdb: