Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MTIDVAAMTRCLKTLSDQTRLIMMRLFLEQEYCVCQLVDMFEMSQPAISQHLRKLKNAGFVNEDRRGQWRYYSINGSCPEFDTLQLILHQIDQEDELLNHIKQKKTQACCQ 1SMT Chain:A ((24-121)) ELQAIAPEVAQSLAEFFAVLADPNRLRLLSLLARSELCVGDLAQAIGVSESAVSHQLRSLRNLRLVSYRKQGRHVYYQLQ----------------DHHIVALYQNALDHLQEC--

General information: TITO was launched using: RESULT: Template: 1SMT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 251 -52655 -209.78 -566.18 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -209.78 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_1SMT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SMT-query.scw PDB file : Tito_Scwrl_1SMT.pdb: