Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSMILAVFIFLLTLVLVIWQPKNLSIGWSACGGAVLALIAGVVNFHDVLTVTGIVWNATLAFVAIILISLILDNIGFFEWAALHMAKAAKGYGVRMFVYVSLLGAIVAALFANDGAALILTPIVLAMVRALHFNEKL--VFPFIIASGFIADTTSLPFVVSNLVNIVSADYFHITFIDYASRMVVPYLFSLLASIIVLYLFFRKSIPKRYDLTEVKKPVEAIKDQNMFRLSWYILGLLLIGYFASEFFS-------IPVSVVAGSIAIIFLIAAQKSPAVHTKKVVKEAPWAIVFFSIGMYVVVYGVRNAGLTDVLSDVIQAAADQG-LFAGTIGMGFIAAILSSIMNNLPTVMIDALAIAGTDT-HGMMREALIYANVIGSDLGPKITPIGSLATLLWLHVLSHKGMKISWGTYFKTGIILTIPTLLITLVGLYIWLLIIHSCF 4F35 Chain:D ((32-448)) ---VVLGISMLAFIAVLWLTEALH--VTVTAILVPVMAVFFGIFETQAAL--NNFANSIIFLFLGGFALAAAMHHQGLDKVIADKVLAMAQGKMSVAVFMLFGVTALLSMWISNTATAAMMLPLVLGVLSKVDADKQRSTYVFVLLGVAYSASIGGIATLVGSPPNAIAAAEVGLSFTDWMKFGLPTAMMMLPMAIAILYFLL----------------VNWDKGKVV---TLGIFGLTVFLWIFSSPINAALGGFKSFDTLVALGAILMLSFA----RVVHWKEIQKTADWGVLLLFGGGLCLSNVLKQTGTSVFLANALSDMVSHMGIFVVILVVATFVVFLTEFASNTASAALLIPVFATVAEAFGMSPVLLSVLIAVAAS-CAFMLPVATPPNAIVFA----SG-HIKQSEMMRVGLYLNIACIGLLTAIAMLFW-------

General information: TITO was launched using: RESULT: Template: 4F35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2167 -424596 -195.94 -1083.15 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain D : 0.73 3D Compatibility (PKB) : -195.94 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_4F35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F35-query.scw PDB file : Tito_Scwrl_4F35.pdb: