Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIKKQDTRRFKEIKPKIMYYGTSTFLLTTLNEDGTTNISPMSSSWALG---HYIILGVGLGGKAIDNLERHKECVINLPGPDLWENVERISSYSGKKSIPPLKKQIGFTYKKEKYEAAGLTPLQSKTVSPTRIKECPIQIEAEVKHIRLPEYESSFAIVETQALHFHAEESIILDENHINPSKWSPLIY--NFRHYFGLGREVGKTFRSET 2D5M Chain:A ((1-184)) ---------MKKSLGARTLAYPTPLFLVGTYDRDSRPNIMAAAWAGICCSQPPSIAVSLRKATYTYRSITERGAFTISIPSRAYVRHADYAGIYSGEN--------------EDKFASLGLTPVPGEHVDAPYVGEFPMAIELKLIHQIE------HTQFIGEIMDVKVDESCLRDDGLPDINKVDPVIFAPVSREYYAVGEFLAKAFSA--

General information: TITO was launched using: RESULT: Template: 2D5M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 899 -83225 -92.58 -472.87 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -92.58 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.378

(partial model without unconserved sides chains): PDB file : Tito_2D5M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2D5M-query.scw PDB file : Tito_Scwrl_2D5M.pdb: