Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIRNPFKDKYYSHDRRALNMLALRVPGLAFILMIYIASIVLQFVSGGWSILLLYAFTILIAIFALLHWHSYRWVKKRVILYFAVQGLITFALANLMTGFFILVIIGLYAFLIGQIIGMADRRRTFLILYLLLLLVINSAYHLHKGEVLHFIVIAAPIMIVIITYAATFFAQVDEKIKAQLTLERLELAHQQVEQLTLQNERQRMARDLHDTLAQGLVSLNMQLDAIHVHLAKGNTERAKEIIQQSMKRVKSTIADARSAIDDLRS-KSEEIGV-LKERITSLMDHFIESTGMACLL-DYRLHQVLDVRTAENCYYIIGECMTNAAKHAEAKTIWISI---WDDEKGRLHLTVKDNGKGFDVEKGKKKRGHYGLLGIQERVRAINGQFNIKSTKLKGTQIEITVPIQGEMQDE 3EHJ Chain:A ((15-214)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ANERIAELVKLEERQRMARDLVDTLGQKLSLMGLKSDLARKLIYKDPEQAAREL--------KSVQQTARTSLNEVRKIVSSMKGIRLKDELINIK-QILEAADIMFIYEEEKWPENISLLNENILSMCLKEAVTNVVKHSQAKTCRVDIQQLWKE----VVITVSDDGT-FKGEENSF---H-GLLGMRERLEFANGSLHIDTE--NGTKLTMAIP--------

General information: TITO was launched using: RESULT: Template: 3EHJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 708 -68692 -97.02 -359.64 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -97.02 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_3EHJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EHJ-query.scw PDB file : Tito_Scwrl_3EHJ.pdb: