Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------MRAIIKFKWAIAAI-------VLALTVVLSLFSPNLTELANQK---GQAQLPADAVSERANAILKQAGEDNNSISVVFTLD---NAIKKETENQLRIIIDKIKKIDGVEEVTSPLSAEKEVKDQLMSKDKKTVLMPVTITGSDKKAEKIADEIYQIV---------------------------PDDLTAY-----ITGASLINQDFAHSSEE--------GLKKTEVIT-------------VCLIIGL--LLIVFRSVVTPFIPIVVVGFSYLIS-QSILGILVY------------NVDFPISTFTQTFLVAILFGIGTD---YCILLLTRFREELANGHDKKEAALIAYRTGGKTLFISGFAVLIGFSALGFAKFAIFQSAVGVAVGVGILMIILYTLLPLFMVTLGEKLF-----WPSKKVLSHSDNKL-WAFLGRHSVARPFLFIV-----ITVVITLPFILTYDDQISFDSTAEIS-----SDYKSIKALEAIKDGFGEGKAFPINVVVKGDKDLTTADTIPYLGNISKAIEKVDHVDS-----------VMTITQPTGKKIKDLYIDNQLGSVSDGLDKTVKGIADV--QSGLTDIENGLNQMAGQTGSASNGGSGGSLGDAADGLGKINQQLQLVSKQISQTGNTAQTVQQL----------------TAISGQLGQIQTGLEQANQQLSGQQAQAGTLTESLKKLSEGVKSANEGLTKVSDGITASSDILEDMSKSPTVRDTGIFIPDQVMKDKDFK---KSIDQYSFADGKGVQLSVVLDSNPYSEQAITTINQIKKAVANEVDGTPLEN--AQIVYGGVTSMNADL-----------KELSTTDFSRTMVIM---IIGLFIVLTILFRSMIMPIYMIASLLLTYYTSIS-ITELIFVNGLGNAGVSWAVPFF---SFVILIALGVDYSIFLLDRFKEEVHLG---IEQGVVRSMSKMGSVIITAAIILAGTFAAMMPSGVNTLMQVASVIIIGLLLYGLVILPLFIPAIIATFGEGNWWPFGRKKGKE 4MT1 Chain:A ((2-1040)) AKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTITLHAIYPGASAQVMEGSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENLAQVEVQNKLS-------------EVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNVVPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNIQISAGSIGSLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNIYLKDVAKVGLGMEDYSSSTRLNGVNTTGMAVMLSNSGNAMATAKAVKERLAVLEKYFPQGMSWKTPYDTSKFVEISIEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLG-GFAFISYMGMSINVLTMFAMILVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISVFVPLAMFSGAAGNIYKQFALTMASSIAFSAFLALTLTPALCATGFFGWFNKKFDSWTHGYEGRVAKVLRK----TFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQGFVMVSVQLPAG--ATKERTDATLAQVTQLA-KSIPEIENIITVSGFSFSGSGQNMAMGFAIFKDWNERTASGSDAVAVAGKLTGMMMGTLKDGFGIAVVPPPILELGSGLSINLQDRNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISFADIRTALASALSS-SYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPLSTIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMEAVQKMVDELGGGYSFEWGGQSSEEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVTGRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAALEAARLRFRPIIMTSFAFILGVVPLYIAAGASSASQRAIGTTVFWGMLVGTLLSVFLVPLFYVVVRKFF----------

General information: TITO was launched using: RESULT: Template: 4MT1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4019 -428465 -106.61 -502.30 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -106.61 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_4MT1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MT1-query.scw PDB file : Tito_Scwrl_4MT1.pdb: