Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMIIIPNNEIAKHMLTDFFAKHWGTPEMAISSGIFRCDELD-----GFAAVNESGEIIGCI----TYTIDGKDCEIISLDSMIENKGIGTALLQQVEEKAKHAHCQRIKLIT-----------------TNDNVNAIA--------FYQKRGYQFAAVFPNAVEKARRLKPEIPEVAENGILIRDEILFSKVID 2A4N Chain:A ((9-161)) ------NNPVLKDQLSDLLRLTW--PEEYGDSSAEEVEEMMNPERIAVAAVDQD-ELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASRGGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPNA--------------------------------

General information: TITO was launched using: RESULT: Template: 2A4N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 392 -63044 -160.83 -529.78 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -160.83 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_2A4N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A4N-query.scw PDB file : Tito_Scwrl_2A4N.pdb: