Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEISDIIYGQHHIDGVLEELIKSAPVQRLKGIYQGGASFLVNRKWNVTRYEHSIGVMLLIKKLGGTI---------------EEQIAGLLHDVSHTAFSHVVDVVFE--------NQAEDYHENIFQQVIVHSEIPDILQKHGYHTEE----LLSDDSR-----------------WTLLEQPAPELCADRTDYTLRDMYRYGHINLHEAQTFLDHLIVRN----GRMFPDS--IEAAEWFVSVYYKEVIDFFLNPVNVYGYEYLARALKAALRH---------------DVISAEDLL-KTDQEVLNILRASKN--EEVLSLLTSIHPGIQVIEDDIQYDFHQKKKMRLIDPSIFLDDKWIKSSGVSEKVRKMGEAAYQKAKKGVYIKILKQ 4MZ7 Chain:A ((29-363)) KVINDPIHGHIELHPLLVRIIDTPQFQRLRYIKQLGGGYYVFPGASHNRFEHSLGVGYLAGCLVHALGEKQPELQISERDVLCVQIAGLCHDLGHGPFSHMFDGRFIPLARPEVKWTHEQGSVMMFEHLINSNGIKPVMEQYGLIPEEDIYFIKEQIVGPLELWPYKGRPENKSFLYEIVSNKRNGIDVDKWDYFARDCHHLGIQNNFDYKRFIKFARVCEVDNELRICARDKEVGNLYDMFHTRNSLHRRAYQHKVGNIIDTMITDAFLKADDYIEITGAGGKKYRISTAIDDMEAYTKLTDNIFLEILYSTDPKLKDAREILKQIEY----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4MZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1261 -149576 -118.62 -573.09 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -118.62 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_4MZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MZ7-query.scw PDB file : Tito_Scwrl_4MZ7.pdb: