Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MNFKVFLLAASTIAVGLVELIVGGILPQIANDL--------DISIVSAGQLISVFALGYAVSGPLLLALTAKIERKRLYLIALFVFFLSNLVA------------------YFSPNFATLMVSRVLAAMSTGLIVVLSLTIAPKIVAPEYRARAIGIIFMGFSSAIALGVPLGILISDS--------FGWRILFLGIGLLALISMLIISI-------------------FFERIP-----AEKMIPFREQLKTIGNLK----------IASSHLVTMFTLAGHYTLYAYFAPFLEETLHLSSFWVSICYFLFGISAVCGGPFGGALSDRLG----SFKSILLVTGSFAIIMFLLPLSTSSMIFFLPVMVIWGLLSWSLAPAQQSYLIEIAPDSSDIQQSFNTSALQVGIALGSAIGGVVLDQTGTVVSTAWCGGSIVIIAV--LFAFISLTRPVQTAKKSSL-------- 4GC0 Chain:A ((5-479)) YNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVAPQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGSAWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLYTVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCMGVLAALFMWKFVPETKGKTLEELEALWE

General information: TITO was launched using: RESULT: Template: 4GC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2028 -329159 -162.31 -848.35 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -162.31 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_4GC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GC0-query.scw PDB file : Tito_Scwrl_4GC0.pdb: