Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTILAIDTSNYTLGIALLREDTVIAEYITYLKKNHSVRAMPAVHSLLNDCDMAPQDLSKIVVAKGPGSYTGVRIGVTLAKTLAWSLDIPISAVSSLETLAANGRHFDGL--ISPIFDARRGQVYTGLYQYKNGLL-----EQVVPDQNVML-----ADWLE-------MLKEKDRPVLFLGHDTSLHKQMIEDVLGTKGFIGTAAQHNPRPSELAFLGKEKEAADVHGLVPDYLRLAEAEAKWIESQK 4Y0W Chain:B ((9-203)) -TLLALDTSTEACSVALLHEGRALSHY-EVI--LHAQRLLPMVRDLLDEAGVALSAVDAIAFGRGPGAFTGVRIAIGVVQGLAFALQRPVLAVSDLAILAQRAYREQGAERVAAAIDARMDEVYWGCYQLQQGEMRLAGSEAVLPPERVAVPWDAAADWFGAGTGWGYVERMPQRPVAL---DASLLPHA-EDLLSLAGF------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4Y0W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 970 -120437 -124.16 -696.16 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -124.16 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.330

(partial model without unconserved sides chains): PDB file : Tito_4Y0W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y0W-query.scw PDB file : Tito_Scwrl_4Y0W.pdb: