Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFIKRLEDAYETLLGNYPAGVSSTSTSKYNEIRKIVSEAFLIGENEVYVTGTSRRISNLDTRFAQGNQRNKHTRMAVAFISIPSVDDSELDELIIRTRNSAITTSSKFCNGEERGTIFDGILLFLVFEGETKVYPLA------FLVFE--NDFELKEKAEELIP--GIEL-KEYPRANQSPAQENNKSAKNE------DEESAKSYVV-------FLDIEEDGSIVEFVEDKDKTYRIG-DMIWTASHTNGSSAITRRLEVIEVVENLVVCKIKHKYNEPVDKNSLLKFVNIEQDLISFLDLHPNVQNGSEGFVSGDIVDENATTSSDDLPEDFENN-------------------------------------- 4A9A Chain:B ((2-369)) STTVEKIKAIEDEMAR--------TQKNKATSFHLGQLKAKLAKLRRELLTSASSGSGGGA---GIGFDVARTGVASVGFVGFPSVGKSTLLSKLTGT-ESETTLVTVPGVIRYKGAKIQMLDLPGIIDGGKQVIAVARTCNLLFIILDVNKPLHHKQIIEKELEGVGIRLNKTPPDILIKKKEKGGISITNTVPLTHLGNDEIRAVMSEYRINSAEIAFRCDATVDDLIDVLEASSRRYMPAIYVLNKIDSLS--IEELELLYRIPNAVPISSGQDWNLDELLQVMWDRLN-------LVRIYTKPKGQIPDFTDPVVLRSDRCSVKDFCNQIHKSLVDDFRNALVYGSSVKHQPQYVGLSHILEDEDVVTILKK

General information: TITO was launched using: RESULT: Template: 4A9A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1141 -97501 -85.45 -333.91 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -85.45 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_4A9A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4A9A-query.scw PDB file : Tito_Scwrl_4A9A.pdb: