Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTHDLHEKTKHYISIYAELKTKLKWKVSHDQILMLISSAYIVNKR--EFDFQRFYDLSSYIKSNIGSFSTLNSHHRFTVASILDIHFQHEAKQTFQTFIDVYNEMVKLGYKRDMFTYLSALILLTGKSETTNQKEQMNMGLAVYQQMKKNHYFLTSTQNVPLAVLLGENGKGLQALQKAETCYQLLAANGFKKGQYLHQVSHILALQSEKEPEMLVSACKQIYQSITESVKKTKDYHYPDLALLTFLEEPDIKTVLCITDELNQEKAFKWQKEMNFKIAVSLYLSEHMEKNLLMESGLYTAIETVIQAQQAAATAAIISSTAASHTHDGN 1DLI Chain:A ((59-133)) -------------LSIKATLDSKAAYKEAE---LVIIATPTNYNSRINYFDTQ-------HVETVIKEVLSVNSHATLIIKSTIPIGFITEMRQKFQT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1DLI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 216 -35637 -164.99 -488.18 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -164.99 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_1DLI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DLI-query.scw PDB file : Tito_Scwrl_1DLI.pdb: