Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEAYKNAGVDIEAGYEAVKRMKKHVERTKRLGVMGSLGGFGGMFDLSELSYQKPVLISGTDGVGTKLKLAFSMDKHDTIGVDAVAMCVNDVLAQGAEPLFFLDYLAVGKADPVKIEQIVQGVAEGCEQSGSALVGGETAEMPGLYTADEYDIAGFSVGVAEKDEIVTGEKIEEGHLLIGLSSSGLHSNGFSLVRKVLLDDAELDLDTTYEPFERPLGEELLEPTRIYVKPVLAAVKSGKIDGMAHVTGGGFIENIPRMLPEGLSAEIDHGSWPIPPIFSFLQEYGKLKEEDMFNVFNMGIGFVLAVKEEHLTDVIGTLESHGEKAYLIGRVKKGEGVTFGGAALS 2Z01 Chain:A ((17-342)) ----------------QAVALMKEHVQKTMRPEVLG-----GGLFDLSALGYRQPVLISGTDGVGTKLKLAFLLDRHDTIGIDCVAMCVNDIIVQGAEPLFFLDYIACGKAVPEKIAAIVKGVADGCVEAGCALIGGETAE--------EYDLAGFAVGVAEKERLITGETIQAGDALVGLPSSGLHSNGYSLVRRIVFEQAKLSLDEIYEPLDVPLGEELLKPTRIYAKLLRSVRERFTIKGMAHITGGGLIENIPRMLPPGIGARIQLGSWPILPIFDFLREKGSLEEEEMFSVFNMGIGLVLAVSPETAAPLVEWLSERGEPAYIIGEVAKGAGVSFAG----

General information: TITO was launched using: RESULT: Template: 2Z01.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1792 -222966 -124.42 -712.35 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -124.42 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.599

(partial model without unconserved sides chains): PDB file : Tito_2Z01.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z01-query.scw PDB file : Tito_Scwrl_2Z01.pdb: