Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYNEGVTSPSQLARKKNATDKWHGSSIRKILENPHYTGTLQQCREYRPSVTSKRRRSVNLENQIIIEKSHEPIIPLEDFLIVQEILITRKRRRPQAEVHLFTNTAVCKDCRRSMHFKKNRKGYVCGSYNKHGLKACSDHYVSEIDLTDKVITNLNKIYLKFSKENYFKELS------EKALKYKEKIESKIIEINEKLNDKKRDKSNLVISLANGVISKEDYQLAINITNED------ISNFETTLHQLSKDLEFQKIEKEIIDFKKSLDKFMKEGTLTPEMLHLLVDEIEVHANGTIEINYRFREPTVPSA 4DVZ Chain:A ((548-717)) ----------------------------------------------------------------------------------------------------LNNLAITSFVRRNLENKLTAKGLSLQEANK----LIKDFLSSNKELAGKAL-NFNKAVAEAKSTGNYDEVKKAQKDLEKSLRKREHLEK---EVEKKLESKSGNK-----KAQANSQKDEIFALINKEANRDARAIAYTQNLKGIKRELSDKLE--KISKDLKDFSKSFDEF----------------------------------------

General information: TITO was launched using: RESULT: Template: 4DVZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 295 2395 8.12 16.52 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 8.12 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.332

(partial model without unconserved sides chains): PDB file : Tito_4DVZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DVZ-query.scw PDB file : Tito_Scwrl_4DVZ.pdb: