Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRKWNNIKEKKASKDANTSRIYAKFGREIYVAAKQGEPDPESNQALKVVLERAKTYSVPKNIIERAIEKAKGGAEENYDELRYEGFGPNGSMIIVDALTNNVNRTAPEVRAAFGKNGGNMGVSGSVAYMFDATAVIGVEGKTADEAL-EILMEADVDVRDILEEDDSAI-VYAEPDQFHAVQEAFKNAGVEEFTVAELTMLAQSEVTLPDDAKEQFEKLIDALEDLEDVQQVYHNVDLGE 4F3Q Chain:A ((9-244)) ---KWANIKHAKARQDAKRGKVFTKLIREITVAARLGGEDIDSNPRLRAVVDKAFAANMPKDTITRAIKRGA----DNLVEVRYEGYGPSGVAVMVDCLTDNKNRTVAEVRHAFSKCDGNLGTEGSVAYLFKQRGLITFPPNSDEEKIMEIALE--VGAEDVTTNDDGSIDVTTLPEDFEKIRNAMKAADLNP-SHAEVTVLASTEVGLDKDSAEQMLRLTEMLEDLDDVQNVYSNADYPE

General information: TITO was launched using: RESULT: Template: 4F3Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 953 -86547 -90.81 -377.93 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -90.81 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.593

(partial model without unconserved sides chains): PDB file : Tito_4F3Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F3Q-query.scw PDB file : Tito_Scwrl_4F3Q.pdb: