Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAFKIENETIADGF-----YACPAVYEDAESITGLLVRTAEWLRDRGSNQWSGLLKGQDIHDITGSIEKGHVFVFKKDE-ELAAVVMLLPAPSEWDRTLWGDDGHEESIYLHRLAVSRRFAGQGLGARVLQWAET-GIHFPEKTRIRLDCVADSDALHSFYRRMGYEFMGADASGYHLFEKEITAE 2OH1 Chain:A ((5-179)) -------NKITAGGLEFLVRFAAPT---DRLKINDLMIDTARWLKESGSTQWSDILHGFDVHNIEQRIELGEVALFETEAGALAGAMIIRKTPSDWDTDLWEDLAIDKAYYLHRIMVSRAFSGISLSKQMIYFAEKLGIEMSVPF-IRLDCIESNETLNQMYVRYGFQFSGK-KNGFYLYQKELSQK

General information: TITO was launched using: RESULT: Template: 2OH1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 707 -95480 -135.05 -568.33 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -135.05 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_2OH1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OH1-query.scw PDB file : Tito_Scwrl_2OH1.pdb: