Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEPVNQPVREAPVFERKKAGRVSPKRILFHVFTATLAVLLLYPVIWLFVSSFKESASIFTTSHSLIPDPFILSNYAEGWKGI----------AGQPFLTFIKNSAIIVGLSTIGAVMSSAVIAYGFARIPFKGKKFWFACMMGTLMLPHEVLMIPQYIMFAKLDW------LNSFKPIVVPQFFGHAFFIFLMIQFIRTIPEELDEAARIDGCGRFACFWRIILPLIAPALATSAIFSFYWKWEELIQPLLYLNKPELYPVSLALKLFLDTESASNWGAMFAMSAVSLLPVILVFFLFQKYIVQGISTTGLK 3RLF Chain:G ((11-295)) -----------------------ARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNFAT---GSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVALSTTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHGGVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVVFILSFIAAITEVPVASLLLRDVNSYTLAVGMQ-QYLNPQNYLWGDFAAAAVMSALPITIVFLLAQRWLVNGLTAGGVK

General information: TITO was launched using: RESULT: Template: 3RLF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 967 -171424 -177.27 -637.26 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain G : 0.75 3D Compatibility (PKB) : -177.27 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_3RLF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RLF-query.scw PDB file : Tito_Scwrl_3RLF.pdb: