Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRMRKKWMALPLAAMMIAGCSHSETSNSASGSKDTIKIMAPLLSPESPSDKSPSLKALEKYTGKEIKVTWVP-DSSYNDKFNIVMASGEMPHAIVIKDKSAGFIKSVKAGAFWELSPYLKDY-KNLSQADE---KILKNS-SVNGEVYGIYRTRDL-IRACMIIRTDWLKNVGLDMPETLDDFYEVLKAFKEKDPDGNGKDDTYGMVVPKWMGLGNGSPWDVLQ-IWFGAP-NRYGVE-NGKLIPDFTTKEYMDALTFFKKLYDEGLINKDFAVMDSAKWNDPVVKGKAGVIVDTGSRASQ--IQSAMEEADESNKDIIDIVGSLEGPNGK-RTF--PTSGYSGMITIPKSSVKTEKELKEVLSFLDKMNDKEAQILTNNGVKGRNYELKDGVFTSLEKNNK---SLL------YEHEGLAQFSMSIPKSEY-YIEDQKTKLFQHRKDIITEGEKIAVFNPAE--SLV---SDVYTQKGAQLDNIILDARTQFIIGEIDEK-GFDDAVELWKKSGGNELMKDLNKLYQSSK 3A09 Chain:A ((8-489)) --------------------------------EKPLTLKIHMHFRDKWVWDENWPVAREVARLTNVKLVGVANRAATNSQEQFNLMMASGQLPDIVGGDNLKDKFIRYGMEGAFIPLNKLIDQNAPNLKAFFKTHPEVQRAITAPDGNIYYLPYVPDGLVSRGYFIRQDWLDKLHLKTPQTVDELYTVLKAFKEKDPNGNGKADEIPFINRDP-EE-VFRLVNFWGARSTGSNTWMDFYVENGKIKHPFAEVAFKDGIKHVAQWYKEGLIDPEIFTRKARSREQTFGNNIGGMT-HD-WFASTALFNDALS--KNIPGFKLVPMAPPINSKGQRWEEDARQIPRPDGWAITATNKNPVE----TIKLFDFYFGPKGRELSNFGVPGLTYDIK-NGKPVYKDTVLKAAQPVNNQMYDIGAQIPIGFW-QDYEYERQWTNDVALQGI---DMYIK---NKYVLPQFTGVNLTVEEREIYDKYWPDVKTYMFEMGQSWVMGTKDPEKTWNDYQQQLKNRGFYQVMIVMQKAYDRQ-

General information: TITO was launched using: RESULT: Template: 3A09.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2356 -145301 -61.67 -322.18 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -61.67 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_3A09.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A09-query.scw PDB file : Tito_Scwrl_3A09.pdb: