Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELVRIFKEHNVFGWISVGTAVLSLLLLNL--------------AIISNVTFYSY------QMLPFAMAAVPFGVVELFIKRGRTGP-GLLGVILNLFVIICVYTIVSVDTNLQFGF 4N4D Chain:A ((29-118)) ---------NTVYGWIIVEIACIPILLLSLRRGLTAGLVGGLIWGILSMITGHAYILSLSQAFLEYLVAPVSLGIAGLF--RQKTAPLKLAPVLLGTFVAV----------------

General information: TITO was launched using: RESULT: Template: 4N4D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 163 -32689 -200.54 -473.75 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -200.54 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.041

(partial model without unconserved sides chains): PDB file : Tito_4N4D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N4D-query.scw PDB file : Tito_Scwrl_4N4D.pdb: