Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTLIILTVLLLSVLTAACSSSSGNQNSKEHKVAVTHDLGKTNVPEHPKRVVVLELGFIDTLLDLGITPVGVADDNKAKQLINKDVLKKIDGYTSVGTRSQPSMEKIASLKPDLIIADTTRHKKVYDQLKKIAPTIALNNLNADYQDTIDASLTIAKAVGKEKEMEKKLTAHEEKLSETKQKISAN-SQSVLLIGNTNDTIMARDENFFTSRLLTQVGYRYAISTSGNSDSSNGGDSVNMKMTLEQLLKTDPDVIILMTGKTDDLDADGKRPIEKNVLWKKLKAVKNGHVYHVDRAVWSLRRSVDGANAILDELQKEMPAAKK 3EIW Chain:A ((8-287)) ---------------------------------ISVKDENGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRII-KPVREKIGDYTSVGTRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTIAKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQFLNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK-LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKEL---------

General information: TITO was launched using: RESULT: Template: 3EIW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1552 -137561 -88.63 -493.05 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -88.63 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_3EIW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EIW-query.scw PDB file : Tito_Scwrl_3EIW.pdb: