Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAVCLVRHGETDWNLQQKCQGKTDIPLNATGERQARETGEYVKDFSWDIIVTSPLKRAKRTAEIINEYLHLPIVEMDDFKERDYGDAEGMPLEERTKRYPD-----------NIYPNMETLEELTDRLMGGLAKVNQAYPNKKVLIVAHGAAIHALLTEISGGDPE-LQSTRLVNACLSNIEFAEEKWRIKDYNINSHLSGFIK 4IJ5 Chain:A ((1-204)) MVKLILVRHAESEWNPVGRYQGLLDPDLSERGKKQAKLLAQELSREHLDVIYSSPLKRTYLTALEIAEAKNLEVIKEDRIIEIDHGMWSGMLVEEVMEKYPEDFRRWVEEPHKVEFQGGESLASVYNRVKGFLEEVRKRHWNQTVVVVSHTVPMRAMYCALLGVDLSKFWSFGCDNASYSVIHMEERRNVILKLNITCHLGEFY-

General information: TITO was launched using: RESULT: Template: 4IJ5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1053 -102393 -97.24 -533.29 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -97.24 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_4IJ5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IJ5-query.scw PDB file : Tito_Scwrl_4IJ5.pdb: