Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYDIISDIHGCYDEMTALIQKLGYTIKNG---------VPVHEEGRVLVFAGDLTDRGPKSIEVIRFVAGAYEKGAVRYVPGNHCNKLYRYLKGNPVKVMHGLETTAAELEELSKDEKKSVSEQFMKLYETAPLYDILHNGELVVAHAGIRADDIGKYTRRVKDFVLYGDVTGETYPDGRPIRRDWAAAYNGKAWVVYGHTPVKEPRKVNRTINIDTGCVFGNQLTGFRFPEIETVSVPSSLPYDESRFRPI 4J6O Chain:A ((12-257)) GPFDIIGDIHGCYDELKMLLEKLGYLIEEVEGGVGSGKYRVTHPEGRKVLFLGDLVDRGPKITEVLKLVMGMVKSGIALCVPGNHDVKLLRKLNGRDVQITHGLDRTLEQLAKEP----QEFIEEVKAFIDGLVSHYVLDDGKLVVAHAGMKEEFQGRGSGKVREFALYGETTGETDEYGLPVRYDWASDYRGKALVVYGHTPQAEVLKVNNTINIDTGCVFGGKLTAYRYPEREIVDVKALKTYYEPAL---

General information: TITO was launched using: RESULT: Template: 4J6O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1301 -154906 -119.07 -653.61 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -119.07 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_4J6O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J6O-query.scw PDB file : Tito_Scwrl_4J6O.pdb: