Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFSKDKLPVILFLFLAGVINYLDRSALSIAAPFIQDDLTLSATQMGLIFSSFSIGYAIFNFLGGVASDR------------YGAKLTLFVAMVVWSLFSGAVALAFGFVSLLIIRILFGMGEGPLSATINKMVNNWFPPTQRASVIGVTNSGTPLGGAISGPIVGMIAVAF--SWKVSFVLIMIIGLIWAVLWFKFVKEKPQ--------ETIKEAPAIKAETSPGE---KIPLTFYLKQKTVLFTAFAFFAYNYILFFFLTWFPSYLVDERGLSVESMSVITVIPWILGFIGLAAGGFVSDYVYK-KTARKGVLFSRKVVLVTCLFS------------SAVLIGFAGLVATTAGAVTLVALSVFFLYLTGAIYWAVIQDVVDQNNVGSVGGFMHFLANTAG-IIGPALTGFIVDQTGTFSGAFLLAGGLAVFASLAVIRFVRPIIGKPAGTEAENPVSY 1PW4 Chain:A ((23-443)) -YRRLRWQIFLGIFFGYAAYYLVRKNFALAMPYLVEQ-GFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLILAAAVMLFMGFVPWATSSIAVM----FVLLFLCGWFQGMGWPPCGRTMV----HWWSQKERGGIVSVWNCAHNVGGGIP-PLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLPPIEEYKND------------TAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWSPTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTIATIVYWMNPAGNPTVDMICMIVIGF-----LIYGPVMLIGLHAL--------------ELAPKKAAGTAAGFTGLFGYLGGSVAASAIVGYTVDFFGWDGGFMVMIGG----SILAVILLIVVMIGEKRRHE-------

General information: TITO was launched using: RESULT: Template: 1PW4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1812 -305485 -168.59 -827.87 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -168.59 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.282

(partial model without unconserved sides chains): PDB file : Tito_1PW4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PW4-query.scw PDB file : Tito_Scwrl_1PW4.pdb: