Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCQSNQIVSHFLSHRNVTNELAEKISKDHYSYKPAETSMSAEELVKHILTSFHLFANVIKEGNASPFQNKQEETETDLNVLAKTYTEKTVAILEQLTEEQLDREIDLTSAFGRKVTGRALLQLAMEHEIHHKGNLFVYVREMGHTELPFYQQRM 3DKA Chain:A ((6-154)) ----NQIVSHFLSHRNVTNELAEKISKDHYSYKPAETSMSAEELVKHILTSFHLFANVIKEGNASPFQN----TETDLNVLAKTYTEKTVAILEQLTEEQLDREID---AFGRKVTGRALLQLAMEHEIHHKGNLFVYVREMGHTELPFYQQR-

General information: TITO was launched using: RESULT: Template: 3DKA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 541 -54197 -100.18 -381.67 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.97 3D Compatibility (PKB) : -100.18 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.97 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_3DKA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DKA-query.scw PDB file : Tito_Scwrl_3DKA.pdb: