Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKGVFSLNYKPKALNKGDTVGVI---APASPPDPKKLDTALLFLEELGLQVKLGKALKNQHGYLAGQDDERLADLHEMFRDDEVKAVLCACGGFGTGRIAAGIDFSLIRKHPKIFWGYSDITFLHTAIHQNTGLVTFHGPMLSTDIGLDDVHPLTKASYKQLF------QETEFTYT------EELSPLTE---LVP-------------GKAEGELVGGNLSLLTSTLGTPFEIDTR-GKLLFIEDIDEEPYQIDRMLNQLKMGGKLTDAAGILVC--DFHNCVPVKREKSLSLEQVLEDYIISAGRPALRGFKIGHCSPSIAVPIGAKAAMNTAEKTAVIEAGVSEGALKT 3TLB Chain:A ((43-362)) ----------------GDTIGFFSSSAPATVTAKNRFFRGVEFLQRKGFKLVSGKLTGKTDFYRSGTIKERAQEFNELVYNPDITCIMSTIGGDNSNSLLPFLDYDAIIANPKIIIGYSDTTALLAGIYAKTGLITFYGPALIPSFG--EHPPLVDITYESFIKILTRKQAGIYTYTLPEKWSDESINWNENKILRPKKLYKNNCAFYGSGKVEGRVIGGNLNTLTGIWGSEWMPEIRNGDILFIEDSRKSIATVERLFSMLKLNRVFDKVSAIILGKHELFDCAGSKRRPYEVLTEVLDGKQI----PVLDGFDCSHTHPMLTLPLGVKLAIDFDNKNISI-----------

General information: TITO was launched using: RESULT: Template: 3TLB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1601 -186639 -116.58 -652.58 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -116.58 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_3TLB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TLB-query.scw PDB file : Tito_Scwrl_3TLB.pdb: