Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MKTLRLNNVTLEMAAYQEESEPKRKIAFTLNVTSETYHDIAVLLYEKTFNVEVPERDLAFRGEMTNYSTSLTNLYEPGAVSEFYIEITEIDKNADS-------- 2JN9 Chain:A ((1-105)) MVKTLRLNNVTLEMAAYQEESEPKRKIAFTLNVTSETYHDIAVLLYEKTFNVEVPERDLAFRGEMTNYSTSLTNLYEPGAVSEFYIEITEIDKNADSLEHHHHHH

General information: TITO was launched using: RESULT: Template: 2JN9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 415 -41203 -99.28 -429.19 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.98 3D Compatibility (PKB) : -99.28 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_2JN9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JN9-query.scw PDB file : Tito_Scwrl_2JN9.pdb: