Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSLLFVYGTLRKHEKNHHLLAQSACINEQARTKGSLFAAKEGPTVVFNDE-DEGYIYGEVYEADELCIHKLDQFFQGYHKQTVFVETDVGIKIALIYFMNKDGCAGFTKISSGDWKEHQMISKSKNPIYYFAYGSCMDNARFQKAGVDHYFQDPVGRAVLKGYTTRFTLKREDGSRADMLEDGGTTEGVLYRIPYSALSYLYKREGVESLTYRPAFVDVEAGGRHYKDCLTFLVLQKEAEIAPPQHYQIEIERGAELYLSPEFTEKLKRHMNSLPKG 2QIK Chain:A ((1-277)) MNSLLFVYGTLRKHEKNHHLLAQSACINEQARTKGSLFA-A--GPTVVFNDEDEGYIYGEVYEADELCIHKLDQFFQGYHKQTVFVETDVGIKIALIYFMN------FTKISSGDWKEHQMISKSKNPIYYFAYGSCMDNARFQKAGVDHYFQDPVGRAVLKGYTTRFTLKREDGSRADMLEDGGTTEGVLYRIPYSALSYLYKREGVESLTYRPAFVDVEAGGRHYKDCLTFLVLQKEAEIAPPQHYQIEIERGAELYLSPEFTEKLKRHMNSLPKG

General information: TITO was launched using: RESULT: Template: 2QIK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1461 -139704 -95.62 -521.28 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.98 3D Compatibility (PKB) : -95.62 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_2QIK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QIK-query.scw PDB file : Tito_Scwrl_2QIK.pdb: