Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEKLAEAHPVWKEEEFGDKDAEDYIISYSMYNGSWLVWEKDGMPVAVSYHLEWSP---SNGKPWLGTVLIDPAEEKKGHAKMIIEQISKLLR-AKHKAMFAGVPIERREWILFLSQCGFEQLKTEKDEK-----GKSFMILVKPLAEAAV 4J3G Chain:A ((76-184)) -----------------------------------VLVAEREGQVVGYASYGPFRPFEGFRHSSEL-SVYVASNARGGGIGRTLLAELIEEARERKVHVLIAGIEAGNAASIALHRSQGFEECGTLKQVGQKFGRWLDLLFMQKI------

General information: TITO was launched using: RESULT: Template: 4J3G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 414 -60658 -146.52 -606.58 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -146.52 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.627

(partial model without unconserved sides chains): PDB file : Tito_4J3G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J3G-query.scw PDB file : Tito_Scwrl_4J3G.pdb: