Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEVGDKIHNTNEQITALEKKKYQIETTLLEKQRDLLKLETQQNKAKLELLF------------ELSEVLTQLEGEEWVSATIALRIIKRNKRKYLDLFDLNDDKAYVNKDKFKFLHDEFFELKQQLNDI 2YD0 Chain:A ((742-825)) -------------------SKYQFSLSSTEKSQIEFALCRTQNKEKLQWLLDESFKGDKIKTQEFPQILT-LIGRNPVGYPLAWQFLRKNWNKLVQKFELGSSS-------------------------

General information: TITO was launched using: RESULT: Template: 2YD0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 206 -7415 -36.00 -102.99 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -36.00 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.630

(partial model without unconserved sides chains): PDB file : Tito_2YD0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YD0-query.scw PDB file : Tito_Scwrl_2YD0.pdb: