Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MTSYDQIWETFLNNCETSDFDVPQQEEDIYKSIRNAILHFNNRLRDNLKADNSTETVNRELSEDDLLI--LAHFLRYIFLLNKKTLFENTWQPFTNDVGIKNFGTQLNSLKQSVMDQKDEIERLILNAAVDYL 2FQ3 Chain:A ((311-395)) SKWFNLEKIHSIEVQSLPEFFTNR--IPSKTPEVYMRYRNFMVN---SYRLNPNEYFSVTTARRNVSGDAAALFRLHKFLTKWGLINYQV------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FQ3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 235 -8970 -38.17 -115.00 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -38.17 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.467

(partial model without unconserved sides chains): PDB file : Tito_2FQ3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FQ3-query.scw PDB file : Tito_Scwrl_2FQ3.pdb: