Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTNKRVLILTANYGNGHVQVAKTLYEQCVRLGFQHVTVSNLYQESNPIVSEVTQYLYLKSFSIGKQFYRLFYYGVDKIYNKRKFNIYFKMGNKRLGELVDEHQPDIIINTFP-MIVVPEYRRRTGRVIPTFNVMTDFC----LHKIWVHENVDKYYVATDYVKEKLLEIGTHPSNVKITGIPIRPQFEES-MPVGPIYKKYNLSPNKKVLLIMAGAHG---VLKNVKELCENLVKDDQ----VQVVVVCGKNTALKESLSALEAENGDKLKVLGYVERIDELFRITDCMITKPGGITLTEATAIGVPVILYKPVPGQEKENANFFEDRGAAIVVNRHEEILESVTSLLA-DEDTLHRMKKNIKDLHLANSSEVILEDILKESEMMTAKQKAKVLS 4X1T Chain:A ((6-389)) ----KKVLILMSDTGGGH----------------------------------------RASAEAIRAAFNQEF--------GDEYQVFIT-------------QPDIIISVHPLMQHVPLRVLRSKGLLKKIVFTTVITDLSTCHPTWFHKLVTRCYCPSTEVAKRAQKAGLETSQIKVYGLPVRPSFVKPVRPKVELRRELGMDENLPAVLLMGGGEGMGPIEATARALADALYDKNLGEAVGQVLIICGRNKKLQSKLSSLDWKI--PVQVKGFITKMEECMGACDCIITKAGPGTIAEAMIRGLPIILNGYIAGQEAGNVPYVVENGCGKFSKSPKEISKIVADWFGPASKELEIMSQNALRLAKPEAVFKIVHDMHELVRKKNS--------

General information: TITO was launched using: RESULT: Template: 4X1T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1766 -19349 -10.96 -63.03 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -10.96 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_4X1T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X1T-query.scw PDB file : Tito_Scwrl_4X1T.pdb: