Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVLHDEKRILTINPGSTSTKIGVFHNERSIFEKTLRHNIEELQRFDRIIDQYEFRKNHILETLHEQGINISKFDAVCARGGLLRPIEGGTYVVNDEMIEDLKSGYAGQHASNLGGIIAREIADGLNIPSYIVDPVVVDEMSVLAKVSGMPEIERKSIFHALNQKAVARKAAASLGKRYENMKMIITHMGGGITIGVHDRGRVVDVNNGLHGEGPFSPERAGTVPAGDLVDLCFSGEYTKEEIMKKLVGTGGLLGYLGTNDAVKVEQMIQGGDEKARFIYDAMAYQVAKEIGAASAALKGEVEAIVLTGGLAYGKSF-VSAIRSYIDWISDVLVYPGENELQSLAQGALRVLQGEEQSKQYRIE 1X9J Chain:E ((3-355)) --------RILTINPGSTSTKLSIFEDERMVKMQNFSHSPDELGRFQKILDQLEFREKIARQFVEETGYSLSSFSAFVSRGGLLDPIPGGVYLVDGLMIKTLKSGKNGEHASNLGAIIAHRFSSETGVPAYVVDPVVVDEMEDVARVSGHPNYQRKSIFHALNQKTVAKEVARMMNKRYEEMNLVVAHMGGGISIAAHRKGRVIDVNNALDGDGPFTPERSGTLPLTQLVDLCFSGKFTYEEMKKRIVGNGGLVAYLGTSDAREVVRRIKQGDEWAKRVYRAMAYQIAKWIGKMAAVLKGEVDFIVLTGGLAHEKEFLVPWITKRVSFIAPVLVFPGSNEEKALALSALRVLRGEEKPKNY---

General information: TITO was launched using: RESULT: Template: 1X9J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1892 -151294 -79.96 -429.81 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain E : 0.87 3D Compatibility (PKB) : -79.96 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_1X9J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1X9J-query.scw PDB file : Tito_Scwrl_1X9J.pdb: