Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKDVKEQLIQVSGPENFDDSNAGRLVYSYDATPQYQSMPDAVIAPRNTDEISRILTICSEHRVPIVPRGSGTNLCGGTCPTEGGLVLLFKHMNQILEIDEENLTATVQPGVITLDMIRAVESKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVTRDYVIGLEVVLANGDIIRTGGKLAKDVAGYDLTRLFVGSEGTLGIVTEAIVKLVPKPETKKTLLALYENIDAAAQTVSDIIA-AKIIPATLEFLDQPTLLVIEDYAKIGLP--TSAKAVLLIEQDGPFETVERDMEKIEAICKKGDA-VSVQTAQTEEEAFALTEARRSALSALARLKPTTILEDATVPRSEIANMVKAINDIAAK--YDISICTFGHAGDGNLHPTCTTD--IRNKDEMERVEQAFAEIFEKAIELGGTITGEHGVGEMKAPYLEMKLKKEGIDAMKALKAAFDPRNILNPGKMFAKDARKRVVAER 3PM9 Chain:A ((14-476)) --SPELIARFTAIVGDKHALTDPLELEAYITEERNLYRGHSPLVLRPGSTEEVVAICKLANEARVALVPQGGNTGLVGGQTPHNGEVVISLKRMDKIREIDTSSNTITVEAGAILQRVQEKAAEVDRLFPLSLGAQGSCTIGGNLSTNAGGTAALAYGLARDMALGVEVVLADGRVMNLLSKLKKDNTGYDLRDLFIGAEGTLGIITAATLKLFPKPRAVETAFVGLQSPDDALKLLGIAQGEAAGNLTSFELIAETPLDFSVRHANNRDPLEARYPWYVLIELSSPRDDARAALESILERGFEDGIVVDAAIANSVQQQQAFWKLREEISPAQKPE-GGSIKHDISVPVAAVPQFIEQANAAVVALIPGARPVPFGHLGDGNIHYNVSQPVGADKAEFLARWHDVSQVVFEVVLRLGGSISAEHGIGVMKRDELAEVKDKTAIELMRSIKALLDPHGIMNPGKVV------------

General information: TITO was launched using: RESULT: Template: 3PM9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2585 -93376 -36.12 -205.22 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -36.12 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_3PM9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PM9-query.scw PDB file : Tito_Scwrl_3PM9.pdb: