Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEIARDVHVKQVKPKKQSSLWWMYIPALLSVLVFMIYPFV--KGTLITFTNWNGFSQVYQWVGFAQYERLFSDPDTWHILKNTLHYGLGSTFFQNVVGLLYALLLNQ--SIKTKAVTRTIVYLPVMISPLIMGYIWYFFFSYDGGALNDLLGVFGISPINALASPSLNPWIIVMINTYQYVGIAMVVYLAGLQSIPKDYYEAAQMDGAKQGQQFFTITLPLLMPSITINIVINIIGGLKL-FDVIIALTAGGPGNASQSMSTFMYDLYFKRQDAGYAATQGIFMAFVILIISFCALAYFKRKETEMS 4TQV Chain:A ((32-320)) ---------------KRDWLLYAMLLPTIIWFLIFLYKPMIGLQMAFKQ-----------PWIGFDHFVTLFQSEQFIRAIKNTLTLSGLSLLFGFPMPILLALMINEVYSKGYRKAVQTIVYLPHFISIVIVAGLVVTFLSPSTGVVNNMLSWIGLDRVYFLTQPEWFRPIYISSNIWKEAGFDSIVYLAAIMSINPALYESAQVDGATRWQMITRITLPCIVPTIAVLLVIRLGHILEVGFEYIILLYQPTTYETADVISTYIYRLGLQGARYDIATAAGIFNAVVALVIVLFA-NHMSRRITK--

General information: TITO was launched using: RESULT: Template: 4TQV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 931 -106384 -114.27 -388.26 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -114.27 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.236

(partial model without unconserved sides chains): PDB file : Tito_4TQV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TQV-query.scw PDB file : Tito_Scwrl_4TQV.pdb: