Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKRLSHLLVPLIAIILGLAAGALIMLVSGYSVASGYSALWNGIFGEIYYVGETIRQITPYILSGLAVAFAFRTGLFNIGVEGQLLVGWTAAVWVGTAFDGPAYIHLPLALITAAAAGGLWGFIPGILKARFYVHEVIVTIMMNYIALHMTNYIISNVLTDHQDKTGKIHESASLRSPFLEQITDYSRLHLGIIVALLAAVIMWFIINKSTKGFELRAVGFNQHASQYAGMSVRKNIMTSMLISGAFAGLAGAMEGLGTFEYAAVKGAFTGVGFDGIAVALLGGNTAVGVVLAACLLGGLKIGALNMPIESGVPSEVVDIVIAIIILFVASSYAIRFVMGKLKKKGAN- 5FN3 Chain:B ((78-467)) GAKHVIMLFVPVTLCMVVVVATIKS--VSFYTRKDG----------QLIY--------TPFTEDTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYKVIHAWLIISSLLL---LFFFSFIYLGEVFKTYNV----AVDYITVALLIWNFGVVGMISIHWKGP----LRLQQAYLIMI---SALMALVFIKYLPEWTAWLILAVISV-TDLVAVLCNE------------TLFPALIYGVKLGLGDFIFYSVLVGKASA---------------TASGDWNTTIACFVAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYL-VQPFMDQLAFHQFYI

General information: TITO was launched using: RESULT: Template: 5FN3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1136 -206079 -181.41 -723.08 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -181.41 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_5FN3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FN3-query.scw PDB file : Tito_Scwrl_5FN3.pdb: