Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAVNFTAFFYNLNISNLTRQVNKMKMDELEKVMIVEGKSDKEKIESVLNEPMRIICTNGTISQLRLEELADELYDKDVYILVDADESGEKLRKQLKREFNEACHLHVDRAYKEVAAAPRHHIASVLLRANLNVHTIFLERKSRGV 2FCJ Chain:B ((2-118)) ---------------------------RRVEKVIIVEGRSDKQKVAAVLNEPVVIVCTNGTISDARLEELADELEGYDVYLLADADEAGEKLRRQFRRMFPEAEHLYIDRAYREVAAAPIWHLAQVLLRARFDVRIESLMR-GRG-

General information: TITO was launched using: RESULT: Template: 2FCJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 514 -81837 -159.22 -699.46 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -159.22 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.625

(partial model without unconserved sides chains): PDB file : Tito_2FCJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FCJ-query.scw PDB file : Tito_Scwrl_2FCJ.pdb: